cycloviolacin O12(38) Protein Card

General Information
Name cycloviolacin O12
Alternative name(s) cycloviolacin T2,varv peptide E
Sequence GLPICGETCVGGTCNTPGCSCSWPVCTRN
Class Cyclotide
Technique MS,Amino acid analysis
Average Mass 2892.28
Monoisotopic Mass 2890.14
m/z M+H 2892.28
ProteinType Wild type
Parent
Organism Viola tricolor
Viola arvensis
Viola baoshanensis
Viola yedoensis
Viola tianshanica
Viola abyssinica
Viola philippica
Viola arcuata
Notes This peptide found in Viola arvensis is also recorded in the UniProtKB entry P83835.
Cyclic Yes

Assay
Cancer Cell Toxicity
Vitri A, varv A and varv E shown to possess cytotoxic activity in cancer cells. [...]Svangard E et al. (2004) J Nat Prod 67:144-7
Anti-HIV
All cyclotides tested from V. yedoensis demonstrated anti-HIV activity, with cycloviolacin Y5 being the most potent. [...]Wang CK et al. (2007) J Nat Prod 71:47-52
Nematocidal
A range of natural variants were selected based on net charge and/or hydrophobicity and were tested alongside the prototypic cyclotide kB1 in larval development assays with H. contortus and T. colubriformis. [...]Colgrave ML et al. (2008) Chembiochem 9:1939-1945

References
Svangard E, Goransson U, Hocaoglu Z, Gullbo J, Larsson R, Claeson P, Bohlin L (2004) Cytotoxic cyclotides from Viola tricolor. J Nat Prod 67:144-7
Craik DJ, Daly NL, Bond T, Waine C (1999) Plant cyclotides: A unique family of cyclic and knotted proteins that defines the cyclic cystine knot structural motif. J Mol Biol 294:1327-36
Goransson U, Luijendijk T, Johansson S, Bohlin L, Claeson P (1999) Seven novel macrocyclic polypeptides from Viola arvensis. J Nat Prod 62:283-6
Ireland DC, Colgrave ML, Craik DJ (2006) A novel suite of cyclotides from Viola odorata: sequence variation and the implications for structure, function and stability. Biochem J. 400:1-12
Wang CK, Colgrave ML, Gustafson KR, Ireland DC, Göransson U, Craik DJ (2007) Anti-HIV Cyclotides from the CHinese Medicinal Herb Viola yedoensis J Nat Prod 71:47-52
Xiang B, Du GH, Wang XC, Zhang SX, Qin XY, Kong JQ, Cheng KD, Li YJ, Wang W (2010) Elucidating the structure of two cyclotides of Viola tianshanica maxim by MALDI TOF/TOF MS analysis Yao Xue Xue Bao 45:1402-1409
Yeshak MY, Burman R, Asres K, Göransson U (2011) Cyclotides from an Extreme Habitat: Characterization of Cyclic Peptides from Viola abyssinica of the Ethiopian Highlands J Nat Prod [ahead]:0-0
Tang J, Wang CK, Pan X, Yan H, Zeng G, Xu W, He W, Daly NL, Craik DJ, Tan N (2010) Isolation and characterization of cytotoxic cyclotides from Viola tricolor. Peptides 31:1434-1440
He,W., Chan,L.Y., Zeng,G., Daly,N.L., Craik,D.J. and Tan,N. (2011) Isolation and characterization of cytotoxic cyclotides from Viola philippica. Peptides 32:1719-1723
Dang,T.T., Chan,L.Y., Huang,Y.H., Nguyen,L.T.T., Kaas,Q., Huynh,T. and Craik,D.J. (2020) Exploring the Sequence Diversity of Cyclotides from Vietnamese Viola Species. J Nat Prod 83:1817-1828

Cross-references
Protein precursor(s) cyclotide precursor 6d
cyclotide precursor 7b
cyclotide precursor 7b
Viola baoshanensis cyclotide precursor 6
Viola baoshanensis cyclotide precursor 7
Viola baoshanensis cyclotide precursor 7
cyclotide precursor 6d
Nucleic acids Viola baoshanensis cyclotide precursor 6 mRNA
Viola baoshanensis cyclotide precursor 7 mRNA
cyclotide precursor 6d mRNA
cyclotide precursor 7b mRNA
Structure
Links GenBank P83836.1
SwissProt P83836

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