MCoTI-II(3) Protein Card

General Information
Name MCoTI-II
Alternative name(s) TI-2
Sequence GGVCPKILKKCRRDSDCPGACICRGNGYCGSGSD
Class Squash trypsin inhibitor
Technique MS,EST,PcR
Average Mass 3452.94
Monoisotopic Mass 3450.49
m/z M+H 3452.94
ProteinType Wild type
Parent
Organism Momordica cochinchinensis
Notes
Cyclic Yes

Assay
Anti-bacterial
MCoTI-II shown to possess no anti-microbial activity. [...]Heitz A et al. (2001) Biochemistry 40:7973-83
Chemical Shift
Chemical shift data for MCoTI-II [...]
NOE Restraint
NOE restraints for 1IB9 [...]
Protease inhibition
Open chain variants have reduced inhibitory activity against bovine trypsin compared to native cyclic proteins. [...]Avrutina O et al. (2005) Biol Chem 386:1301-6

References
Felizmenio-Quimio ME, Daly NL, Craik DJ (2001) Circular proteins in plants: solution structure of a novel macrocyclic trypsin inhibitor from Momordica cochinchinensis. J Biol Chem 276:22875-82
Heitz A, Hernandez JF, Gagnon J, Hong TT, Pham TT, Nguyen TM, Le-Nguyen D, Chiche L (2001) Solution structure of the squash trypsin inhibitor MCoTI-II. A new family for cyclic knottins. Biochemistry 40:7973-83
Hernandez JF, Gagnon J, Chiche L, Nguyen TM, Andrieu JP, Heitz A, Trinh Hong T, Pham TT, Le Nguyen D (2000) Squash trypsin inhibitors from Momordica cochinchinensis exhibit an atypical macrocyclic structure. Biochemistry 39:5722-30
Thongyoo P, Tate EW, Leatherbarrow, RJ (2006) Total synthesis of the macrocyclic cysteine knot microprotein MCoTI-II. Chem Commun 27:2848-2850
Thongyoo P, Jaulent AM, Tate EW, Leatherbarrow, RJ (2007) Immobilized protease-assisted sysnthesis of engineered cysteine-knot microproteins Chembiochem 8:1107-1109
Cascales,L., Henriques,S.T., Kerr,M.C., Huang,Y.H., Sweet,M.J., Daly,N.L. and Craik,D.J. (2011) Identification and characterization of a new family of cell-penetrating peptides: cyclic cell-penetrating peptides. J. Biol. Chem. 286:36932-36943
Smithies, B.J., Huang, Y.H., Jackson, M.A., Yap, K., Gilding, E.K., Harris, K.S., Anderson, M.A. and Craik, D.J., (2020) Circular permutation of the native enzyme-mediated cyclization position in cyclotides ACS Chemical Biology 15:962-969
Du, J., Yap, K., Chan, L.Y., Rehm, F.B., Looi, F.Y., Poth, A.G., Gilding, E.K., Kaas, Q., Durek, T. and Craik, D.J. (2020) A bifunctional asparaginyl endopeptidase efficiently catalyzes both cleavage and cyclization of cyclic trypsin inhibitors Nature communications 11:1575-0

Cross-references
Protein precursor(s) TIPTOP1
TIPTOP1
TIPTOP1
TIPTOP2
TIPTOP2
TIPTOP2
TIPTOP3
TIPTOP3
TIPTOP3
TIPTOP3
TIPTOP3
TIPTOP5
TIPTOP5
TIPTOP6
Nucleic acids
Structure Solution structure of MCoTI-II, a macrocyclic trypsin inhibitor
SOLUTION STRUCTURE OF THE SQUASH TRYPSIN INHIBITOR MCOTI-II, NMR, 30 STRUCTURES.
trypsin:MCoTi-II complex
Links SwissProt P82409

Tools